{"id":83,"date":"2014-11-18T22:03:45","date_gmt":"2014-11-18T22:03:45","guid":{"rendered":"http:\/\/cmap420.com\/wordpress\/?page_id=83"},"modified":"2015-09-02T19:39:30","modified_gmt":"2015-09-02T19:39:30","slug":"cmap-guide-checklist","status":"publish","type":"page","link":"http:\/\/cmap420.com\/wordpress\/cmap-guide\/cmap-guide-checklist\/","title":{"rendered":"Checklist"},"content":{"rendered":"

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Table of Contents<\/a> \u00a0 \u00a0 Introduction<\/a>\u00a0\u00a0\u00a0\u00a0 Checklist<\/a>\u00a0\u00a0\u00a0\u00a0 Compilation List<\/a>
\nFull View<\/a>\u00a0\u00a0\u00a0\u00a0 Printable Files<\/a> \u00a0 \u00a0 Guest Blog<\/a><\/span><\/strong><\/address>\n

\u00a0<\/strong><\/h3>\n

beta version<\/strong><\/h3>\n

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2.0.0 CMAP\u2122<\/em> Checklist:
\nfor Cannabis Research in Human Subjects (Appendix A)<\/span><\/h1>\n

2.1.0 Purpose of the Study<\/strong>
\n2.1.0.1 Exploratory<\/span>
\n 2.1.0.2 To prove a hypothesis<\/span><\/p>\n

2.2.0 Sponsor<\/strong><\/span>
\n 2.2.0.1 Institution<\/span>
\n 2.2.0.2 Private study<\/span>
\n 2.2.0.3 Not indicated<\/span><\/p>\n

2.3.0 Bias<\/strong><\/span>
\n 2.3.0.1 Sources of funding<\/strong><\/span>
\n 2.3.0.1.1 National Institutes of Health (NIH)<\/span>
\n 2.3.0.1.2 National Institute of Drug Abuse<\/span>
\n 2.3.0.1.3 Pharmaceutical company<\/span>
\n 2.3.0.1.4 Private funding and organizations<\/span>
\n 2.3.0.1.5 Not for profit<\/span>
\n 2.3.0.1.6 Other:<\/span><\/p>\n

2.3.0.2 Affiliations<\/strong><\/span>
\n 2.3.0.2.1 academic<\/span>
\n 2.3.0.2.2 commercial<\/span>
\n 2.3.0.2.3 non-profit<\/span>
\n 2.3.0.2.4 governmental<\/span><\/p>\n

2.3.0.3 Potential undisclosed bias based on:<\/strong><\/span>
\n 2.3.0.3.1 personal public statements<\/span>
\n 2.3.0.3.2 interpretation and conclusions of published and unpublished studies<\/span>
\n 2.3.0.3.3 sources of funding<\/span>
\n 2.3.0.3.4 professional or academic affiliations<\/span><\/p>\n

2.4.0 Institutional Review Board (IRB) approval<\/strong><\/span>
\n 2.4.0.1 IRB run by the organization (academic or commercial) conducting independent research<\/span>
\n 2.4.0.2 IRB run by a company in the business of providing independent IRB services<\/span><\/p>\n

2.5.0 Abstract<\/strong><\/span><\/p>\n

2.6.0 Protocol<\/strong><\/span><\/p>\n

2.7.0 Methodology<\/strong><\/span><\/p>\n

2.7.1 Subject Selection<\/strong><\/span><\/p>\n

2.7.1.1 Sampling technique<\/strong><\/span>
\n 2.7.1.1.1 Controlled sampling<\/span>
\n 2.7.1.1.2 Subjects randomly assigned to a control or test group<\/span>
\n 2.7.1.1.3 Subjects self-selected<\/span>
\n 2.7.1.1.4 Single-blind<\/span>
\n 2.7.1.1.5 Double-blind<\/span><\/p>\n

2.7.1.1.6 Exclusionary criteria in sampling<\/strong><\/span>
\n 2.7.1.1.6.1 age<\/span>
\n 2.7.1.1.6.2 gender<\/span>
\n 2.7.1.1.6.3 ethnicity<\/span><\/p>\n

2.7.1.1.6.4 use or history of use of<\/strong><\/span>
\n 2.7.1.1.6.4.1 prescription drugs<\/span>
\n 2.7.1.1.6.4.2 over the counter drugs<\/span>
\n 2.7.1.1.6.4.3 recreational drugs<\/span>
\n 2.7.1.1.6.4.4 tobacco<\/span>
\n 2.7.1.1.6.4.5 nicotine vape (see Method of Administration, Sections 2.7.3.6.1.1 and 2.7.3.6.1.2)<\/span>
\n 2.7.1.1.6.4.6 nicotine transdermal patch<\/span>
\n 2.7.1.1.6.4.7 alcohol<\/span>
\n
\n2.7.1.1.6.5 other exclusionary criteria<\/strong><\/span><\/p>\n

2.7.1.1.7 Psychophysiological considerations<\/strong><\/span>
\n 2.7.1.1.7.1 Set<\/strong><\/span>
\n 2.7.1.1.7.1.1 mental state<\/span>
\n 2.7.1.1.7.1.2 expectation effect<\/span>
\n 2.7.1.1.7.1.3 double blind<\/span><\/p>\n

2.7.1.1.7.2 Setting<\/strong><\/span>
\n 2.7.1.1.7.2.1 physical environment<\/span>
\n 2.7.1.1.7.2.2 social environment<\/span>
\n 2.7.1.1.7.2.3 double blind<\/span>
\n 2.7.1.1.7.2.4 Examples:<\/span>
\n 2.7.1.1.7.2.4.1 informal by subject’s choice<\/span>
\n 2.7.1.1.7.2.4.2 formal by protocol<\/span>
\n 2.7.1.1.7.2.4.3 “mood” of the setting<\/span>
\n 2.7.1.1.7.2.4.4 experience (history of cannabis use)<\/span>
\n 2.7.1.1.7.2.4.5 subject’s circadian rhythm<\/span>
\n 2.7.1.1.7.2.4.6 other herbal supplements, over-the-counter and prescription drugs)<\/span>
\n 2.7.1.1.7.2.4.7 other active and “inert” ingredients in topicals, et al.<\/span>
\n 2.7.1.1.7.2.5 \u00a0\u00a0 New cultivar effect<\/span>
\n 2.7.1.1.7.2.6\u00a0\u00a0\u00a0 Typical rotation\/use sequence of cultivar re tolerance not just to<\/span> THC, but “entourage” or “integration” effect as well<\/span><\/p>\n

2.7.2 Cannabis Selection<\/strong><\/span><\/p>\n

2.7.2.1 Plant Profile<\/strong><\/span>
\n 2.7.2.1.1 Botanical identification (Cannabis Sativa L.)<\/span><\/p>\n

2.7.2.1.1.1 Hybrids – bred into thousands of cultivars from variations grown from these 4 sub-species)<\/strong><\/span>
\n 2.7.2.1.1.1.1 Sativa<\/span>
\n 2.7.2.1.1.1.2 Indica<\/span>
\n 2.7.2.1.1.1.3 Ruderalis<\/span>
\n 2.7.2.1.1.1.4 Afghanica<\/span><\/p>\n

2.7.2.1.1.2 Cultivar name<\/strong> (e.g., “Super Lemon Haze”)<\/span>
\n 2.7.2.1.1.2.1 Cultivar’s genetic lineage: (e.g., Lemon Skunk x Super Silver Haze)<\/span>
\n 2.7.2.1.1.2.2 validation of cultivar<\/span>
\n 2.7.2.1.1.2.3 Characterization of effects of cultivar studied<\/span><\/p>\n

2.7.2.1.2 Cultivation<\/strong><\/span><\/p>\n

2.7.2.1.2.1 Growth conditions<\/span><\/p>\n

2.7.2.1.2.1.1 Grower-producer<\/span><\/p>\n

2.7.2.1.2.1.2 Phenotype<\/span>
\n 2.7.2.1.2.1.2.1 seed<\/strong><\/span>
\n 2.7.2.1.2.1.2.1.1 genetics verified<\/span>
\n 2.7.2.1.2.1.2.1.2 genetics not verified<\/span>
\n 2.7.2.1.2.1.2.2 clone<\/strong><\/span>
\n 2.7.2.1.2.1.2.2.1 genetics verified<\/span>
\n 2.7.2.1.2.1.2.2.1 genetics not verified<\/span>
\n 2.7.2.1.2.1.2.3 indoor<\/strong><\/span>
\n 2.7.2.1.2.1.2.3.1 type of lighting<\/span>
\n 2.7.2.1.2.1.2.3.1.1 LEDs (specific spectrum)<\/span>
\n 2.7.2.1.2.1.2.3.1.2 high pressure sodium (HPS)<\/span>
\n 2.7.2.1.2.1.2.3.1.3 metal halide (MH)<\/span>
\n 2.7.2.1.2.1.2.4 outdoor<\/strong><\/span>
\n 2.7.2.1.2.1.2.4.1 sungrown<\/span>
\n 2.7.2.1.2.1.2.5 greenhouse<\/strong><\/span>
\n 2.7.2.1.2.1.2.6 hydroponic<\/strong><\/span>
\n 2.7.2.1.2.1.2.7 soil<\/strong><\/span>
\n 2.7.2.1.2.1.2.8 nutrients<\/strong><\/span>
\n 2.7.2.1.2.1.2.9 water quality<\/strong><\/span>
\n 2.7.2.1.2.1.2.9.1 tap water<\/span>
\n 2.7.2.1.2.1.2.9.2 alkaline\/ph balance<\/span>
\n 2.7.2.1.2.1.2.9.3 bottled<\/span>
\n 2.7.2.1.2.1.2.9.4 hard<\/span>
\n 2.7.2.1.2.1.2.9.5 soft<\/span>
\n 2.7.2.1.2.1.2.10 organic<\/strong><\/span>
\n 2.7.2.1.2.1.2.11 not organic<\/strong><\/span>
\n 2.7.2.1.2.1.2.11.1 pesticides used<\/span>
\n 2.7.2.1.2.1.2.12 wash-out period (flush)<\/strong><\/span><\/p>\n

2.7.2.1.2.1.3 Living Plant Testing<\/strong><\/span><\/p>\n

2.7.2.1.3 Harvest<\/strong><\/span>
\n 2.7.2.1.3.1 harvest time<\/strong><\/span>
\n 2.7.2.1.3.1.1 readiness of trichomes<\/span>
\n 2.7.2.1.3.1.2 days into flowering<\/span>
\n 2.7.2.1.3.1.3 time of day of actual harvest<\/span><\/p>\n

2.7.2.1.3.2 Testing at harvest<\/strong><\/span><\/p>\n

2.7.2.1.4 Post-harvest<\/strong><\/span><\/p>\n

2.7.2.1.4.1 Fresh<\/strong><\/span>
\n 2.7.2.1.4.1.1 raw<\/span>
\n 2.7.2.1.4.1.2 cooked<\/span><\/p>\n

2.7.2.1.4.2 Trim<\/strong><\/span><\/p>\n

2.7.2.1.4.3 Drying<\/strong><\/span>
\n 2.7.2.1.4.3.1 moisture content<\/span><\/p>\n

2.7.2.1.4.4 Cure<\/strong><\/span>
\n 2.7.2.1.4.4.1 testing<\/span><\/p>\n

2.7.2.1.4.5 Storage<\/strong><\/span>
\n 2.7.2.1.4.5.1 oxygen<\/span>
\n 2.7.2.1.4.5.2 light<\/span>
\n 2.7.2.1.4.5.3 heat<\/span>
\n 2.7.2.1.4.5.4 time<\/span>
\n 2.7.2.1.4.5.5 humidity<\/span>
\n 2.7.2.1.4.5.6 biological sterilty<\/span><\/p>\n

2.7.2.2 Processing<\/strong><\/span><\/p>\n

2.7.2.2.1 Whole Plant<\/strong><\/span>
\n 2.7.2.2.1.1 Flower<\/span>
\n 2.7.2.2.1.2 Trim
\n2.7.2.2.2 Extraction<\/strong><\/span>
\n 2.7.2.2.2 Solvent<\/strong><\/span>
\n 2.7.2.2.2.1 CO2<\/strong><\/span>
\n 2.7.2.2.2.2 ice water<\/strong><\/span>
\n 2.7.2.2.2.3 butane<\/strong><\/span>
\n 2.7.2.2.2.3.1 purge<\/span>
\n 2.7.2.2.2.3.1.1 type of purge<\/span>
\n 2.7.2.2.2.3.1.2 test of purge results<\/span>
\n 2.7.2.2.3 Solution for extraction of fat soluble molecules<\/strong><\/span>
\n 2.7.2.2.3.1 butter, oil, or fats<\/strong><\/span>
\n2.7.2.2.3.1.1 plant
\n<\/span>2.7.2.2.3.1.1.1 saturated<\/span>
\n2.7.2.2.3.1.1.2 unsaturated
\n2.7.2.2.3.1.2 animal
\n2.7.2.2.3.1.2.1 saturated
\n2.7.2.2.3.1.2.2 unsaturated
\n<\/span>2.7.2.2.3.2 food grade ethanol<\/strong><\/span>
\n 2.7.2.2.3.2.1 alcohol content\/evaporated<\/span>
\n 2.7.2.2.3.2.2 alcohol content\/not evaporated<\/span>
\n 2.7.2.2.3.3 food grade glycerin<\/strong><\/span>
\n 2.7.2.2.3.3.1 glycemic load<\/span>
\n 2.7.2.2.3.4 food grade propylene glycol<\/strong><\/span><\/p>\n

2.7.2.3 Laboratory Analysis<\/strong><\/span><\/p>\n

2.7.2.3.1 Cannabinoid profile (partial)<\/strong> – see Glossary and Other Resources, Section 1.4.1.3.7<\/span><\/p>\n

2.7.2.3.1.1\u00a0\u00a0 CBG-A (Cannabigerol-acid)<\/span>
\n 2.7.2.3.1.2\u00a0\u00a0 CBG \u00a0\u00a0 (Cannabigerol)<\/span>
\n
\n2.7.2.3.1.3\u00a0\u00a0 THC-A (tetrahydrocannabinol-acid)<\/span>
\n 2.7.2.3.1.4\u00a0\u00a0 THC (tetrahydrocannabinol, THC, \u03949 , Delta-9- after decarboxylation)<\/span>
\n 2.7.2.3.1.5\u00a0\u00a0 THC 11 (tetrahydrocannabinol – 11)<\/span>
\n 2.7.2.3.1.6\u00a0\u00a0 THCV (Tetrahydrocannabivarin)<\/span><\/p>\n

2.7.2.3.1.7\u00a0\u00a0 CBD-A (Cannabidiol)
\n 2.7.2.3.1.8\u00a0\u00a0 CBD (Cannabidiol – after decarboxylation)<\/span><\/p>\n

2.7.2.3.1.9 \u00a0 CBC-A (Cannabichromene-acid)
\n 2.7.2.3.1.10 CBC (Cannabichromene – after decarboxylation)<\/span><\/p>\n

2.7.2.3.1.11 CBN-A (Cannabinol-acid)
\n2.7.2.3.1.12 CBN (Cannabinol – primarily found as a result of THC breakdown during storage, not from CBN-A oxidation)<\/p>\n

2.7.2.3.1.13 Other cannabinoids<\/p>\n

2.7.2.3.2 Terpene profile (partial)<\/strong>\u00a0 There are more than 100 terpenes with a range of effects — many well studied in other plants; they are a major driver of the “entourage effect” and many terpenes cross the blood-brain barrier.\u00a0 The following list includes some of the major terpenes, other plants in which they are found, and some of their reported effects.<\/span><\/p>\n

2.7.2.3.2.1 Monoterpenoids<\/strong> (highly volatile and likely to be lost or diminished during drying and early storage)<\/p>\n

2.7.2.3.2.1.1\u00a0\u00a0 Borneol (camphor, rosemary, and mint) – anesthetic, anti-inflammatory, analgesic<\/p>\n

2.7.2.3.2.1.2\u00a0\u00a0 Cineole (eucalyptus) – analgesic for topical uses, stimulant<\/p>\n

2.7.2.3.2.1.3\u00a0\u00a0 cis-Ocimene (variety of plants and fruit) – used in perfumery<\/p>\n

2.7.2.3.2.1.4\u00a0\u00a0 Limonene (orange peel) – hypothermogenic<\/p>\n

2.7.2.3.2.1.5\u00a0\u00a0 Linalool (lavender) – relaxant, anesthetic, anti-convulsant, analgesic, anxiolytic, burn therapeutic agent, anti-depressant (via seratonin-receptor transmission amplification)<\/p>\n

2.7.2.3.2.1.6\u00a0\u00a0 Myrcene (hops) – sedative for sleepiness, analgesic, muscle relaxant<\/p>\n

2.7.2.3.2.1.7\u00a0\u00a0 a-Pinene (pine) – bronchodilator, memory retention aid, anti-bacterial, anti-inflammatory; crosses the blood-brain barrier easily.<\/p>\n

2.7.2.3.2.1.8\u00a0\u00a0 Pulegone (rosemary) – may prevent short-term memory loss (acetylcholinesterase protein inhibitor)<\/p>\n

2.7.2.3.2.1.9\u00a0\u00a0 Terpinolene (cumin, lilac, apple, tea-tree and conifers) – anti-septic, anti-bacterial, anti-fungal, preservative, treats insomnia in a blend of lilac and lavender.<\/span><\/p>\n

2.7.2.3.2.1.10 Terpineol (lilac, apple blossoms) – used in perfumes, cosmetics, and flavorings.<\/span><\/p>\n

2.7.2.3.2.1.11 Others<\/p>\n

2.7.2.3.2.2 Sesquiterpenoids (less volatile)
\n<\/strong>
\n2.7.2.3.2.2.1 a-Caryophyllene or Humulene (hops, pine) – anti-inflammatory<\/span><\/p>\n

2.7.2.3.2.2.2 b-Caryophyllene (black pepper) – anti-inflammatory, analgesic, protects cells lining the digestive tract, binds to CB2 receptors<\/p>\n

2.7.2.3.2.2.3 Caryophyllene oxide (oxidated Caryophylene) – anti-fungal, anti-bacterial<\/p>\n

2.7.2.3.2.2.4 b-Eudesmol (Chinese herb) – anti-convulsive<\/p>\n

2.7.2.3.2.2.5 trans-Nerolidol (ginger, jasmine, lavender, tea tree, lemon grass) – flavoring, perfumery, and may enhance skin penetration for transdermal delivery of therapeutic drugs<\/p>\n

2.7.2.3.2.3 Other Cannabis Terpenes<\/strong><\/p>\n

2.7.2.3.3 Flavinoid profile<\/strong><\/span><\/p>\n

2.7.2.3.3.1 Flavones<\/span><\/p>\n

2.7.2.3.3.2 Flavanols<\/span><\/p>\n

2.7.2.3.4 Vanilloid profile<\/strong><\/span><\/p>\n

2.7.2.3.4.1 Vanilloids<\/span><\/p>\n

2.7.2.4.5 Contaminants<\/strong><\/span><\/p>\n

2.7.2.3.6 Lab QA Program and Testing<\/strong> (per Washington State Liquor Control Board – WSLCB)<\/span>
\n Washington State Liquor Control Board (to become WS Liquor and Cannabis Control Board), WAC 314-55-103 “Good Laboratory Practice Checklist.” (for certification of I-502 products), PDF, pp 7-9. http:\/\/www.liq.wa.gov\/publications\/Marijuana\/I-502\/Good_Laboratory_Practice_Certification_Checklist.pdf<\/a><\/span><\/p>\n

2.7.2.3.6.1 Sampling\/sample protocols<\/strong> adequate chain of custody tracking<\/span><\/strong>
\n 2.7.2.3.6.1.1 sample preparation, extraction and dilution (SOP)<\/span>
\n
\n2.7.2.3.6.1.2 demonstration of recovery for samples in various matrices (SOP):<\/strong><\/span>
\n 2.7.2.3.6.1.2.1 plant material\/flower<\/span>
\n 2.7.2.3.6.1.2.2 edibles (solid and liquid meant to be consumed orally)<\/span>
\n 2.7.2.3.6.1.2.3 topicals<\/span>
\n 2.7.2.3.6.1.2.4 concentrates<\/span>
\n 2.7.2.3.6.1.2.5 tincture<\/span>
\n 2.7.2.3.6.1.2.6 spray<\/span>
\n
\n2.7.2.3.6.1.3 Whole plant sampling method (cannabis selection)<\/strong><\/span>
\n 2.7.2.3.6.1.3.1 Location of sample<\/span>
\n 2.7.2.3.6.1.3.1.1 Specific location (bud, leaves, high, low)<\/span>
\n 2.7.2.3.6.1.3.1.1 Several locations averaged<\/span>
\n 2.7.2.3.6.1.3.2 Homogenization<\/span>
\n 2.7.2.3.6.1.3.3 Weighing<\/span>
\n 2.7.2.3.6.1.3.4 Labeling<\/span>
\n 2.7.2.3.6.1.3.5 Sample identifier (source, lot)<\/span>
\n 2.7.2.3.6.1.3.6 Date<\/span>
\n 2.7.2.3.6.1.3.7 Tracking<\/span>
\n
\n2.7.2.3.6.1.4.8 Condition of sample<\/strong><\/span>
\n 2.7.2.3.6.1.4.8.1 macroscopic inspection<\/strong><\/span>
\n 2.7.2.3.6.1.4.8.1.1 foreign matter<\/span>
\n 2.7.2.3.6.1.4.8.2 microscopic inspection<\/strong><\/span>
\n 2.7.2.3.6.1.4.8.2.1 microbial contaminants<\/span>
\n 2.7.2.3.6.1.4.8.2.2 nutrients and fertilizers<\/span>
\n 2.7.2.3.6.1.4.8.2.3 pesticides<\/span>
\n 2.7.2.3.6.1.4.8.2.4 moisture analysis<\/span>
\n 2.7.2.3.6.1.4.8.2.5 residual solvent<\/span>
\n 2.7.2.3.6.1.4.8.2.6 metabolite analysis<\/span>
\n 2.7.2.3.6.1.4.8.2.6.1 beginning of testing<\/span>
\n 2.7.2.3.6.1.4.8.2.6.2 during testing<\/span>
\n 2.7.2.3.6.1.4.8.2.6.3 end of testing<\/span>
\n 2.7.2.3.6.1.4.8.2.7 sample potency<\/span>
\n 2.7.2.3.6.1.4.8.2.7.1 beginning of testing<\/span>
\n 2.7.2.3.6.1.4.8.2.7.2 during testing<\/span>
\n 2.7.2.3.6.1.4.8.2.7.3 end of testing<\/span>
\n 2.7.2.3.6.1.4.8.2.8 chemical assay procedure\/method<\/span>
\n 2.7.2.3.6.1.4.8.2.8.1 method<\/span>
\n 2.7.2.3.6.1.4.8.2.8.1.1 LC<\/span>
\n 2.7.2.3.6.1.4.8.2.8.1.2 HPLC<\/span>
\n 2.7.2.3.6.1.4.8.2.8.1.3 uHPLC (aka UPLC)<\/span>
\n 2.7.2.3.6.1.4.8.2.8.1.4 GC<\/span>
\n 2.7.2.3.6.1.4.8.2.8.1.5 Headspace GC<\/span>
\n 2.7.2.3.6.1.4.8.2.8.1.6 next generation advances<\/span>
\n 2.7.2.3.6.1.4.8.2.8.2 data protocols followed<\/span>
\n 2.7.2.3.6.1.4.8.2.8.2.1 Calculations for quantification of bioactive compound content in various matrices – SOPs<\/span>
\n 2.7.2.3.6.1.4.8.2.8.2.2 Determination of the range for reporting the quantity (LOD\/LOQ) data review or generation<\/span>
\n 2.7.2.3.6.1.4.8.2.8.2.3 Reporting of data: certificates of analysis (CA-clear and standardized format<\/span>
\n 2.7.2.3.6.1.4.8.2.8.2.4 Documentation that value reported in CA is within range\/limits of the analytical method<\/span>
\n 2.7.2.3.6.1.4.8.2.8.2.5 Documentation that qualitative results (below LOQ but above LOD) are reported as “trace”<\/span>
\n 2.7.2.3.6.1.4.8.2.8.2.6 Documentation that method has specificity for degree of quantitation reported<\/span>
\n 2.7.2.3.6.1.4.8.2.8.2.7 Use of appropriate controls: Documentatoin of daily use of positive and neg controls that<\/span><\/p>\n

2.7.2.3.6.1.5 Level II validation of methodology used for quantification of bioactive compounds<\/strong><\/span>
\n 2.7.2.3.6.1.5.1 single lab validation parameters are demonstrated for GC, HPLC data review<\/strong>:*<\/span>
\n 2.7.2.3.6.1.5.1.1 linearity of reference standards<\/span>
\n 2.7.2.3.6.1.5.1.2 use of daily standard curve<\/span>
\n 2.7.2.3.6.1.5.1.3 accuracy<\/span>
\n 2.7.2.3.6.1.5.1.4 precision<\/span>
\n 2.7.2.3.6.1.5.1.5 recovery (5 determinations not less than 90%)<\/span>
\n 2.7.2.3.6.1.5.1.6 reproducibility over time within a relative standard deviation of 5%<\/span>
\n
\n2.7.3 Methods of Extraction (MOE); Method of Administration (MOA); Timing of Administration (TOA)<\/strong><\/span>
\n Note: Cannabis may be pre-activated (heated) or cold-extracted and activated during consumption<\/span><\/p>\n

2.7.3.1 Heated plant<\/strong> – heated sufficiently to activate cannabinoids (decarboxylated)<\/span>
\n 2.7.3.1.1 examples: combust, vape, dab, edible, tincture<\/span><\/p>\n

2.7.3.2 Partially heated plant<\/strong> – not heated sufficiently to activate<\/span>
\n 2.7.3.2.1 (tincture, other low temperature extracts)<\/span><\/p>\n

2.7.3.2.1.1 Raw Plant<\/span>
\n 2.7.3.2.2 examples: juicing, fresh greens in salad<\/span><\/p>\n

2.7.3.3 Extracts\/Concentrates<\/strong><\/span>
\n solvent (if any) may be pharmaceutical grade (PH), food grade (F), Commercial ( C), Industrial (I), organic (O), plant-based (PL), or animal-based (A)<\/span>
\n 2.7.3.3.1 kief<\/strong> – solventless mechanical extraction<\/span>
\n 2.7.3.3.2 hash<\/strong> – solventless, ice water, mechanical extraction<\/span>
\n 2.7.3.3.3 hash oil<\/strong> – solvent extraction<\/span>
\n 2.7.3.3.3.1 butane<\/span>
\n 2.7.3.3.3.2 CO2<\/span>
\n 2.7.3.3.3.3 fats\/oils<\/span>
\n 2.7.3.3.3.4 alcohol\/ethanol<\/span><\/p>\n

2.7.3.4 Ingredients in Edibles and other MOAs<\/strong><\/span>
\n Note: pharmaceutical grade (PH), food grade (F), Commercial ( C), Industrial (I), organic (O), plant-based (PL), or animal-based (A)<\/span>
\n 2.7.3.4.1 fats (butter, oil, etc.)<\/span>
\n 2.7.3.4.2 alcohol\/ethanol (e.g., Everclear)<\/span>
\n 2.7.3.4.3 propylene glycol<\/span>
\n 2.7.3.4.4 glycerin<\/span><\/p>\n

2.7.3.5 Bio-Pharma<\/strong><\/span>
\n 2.7.3.5.1 Pharmaceutical extracts (e.g., Sativex)<\/span>
\n 2.7.3.5.2 Synthesized Cannabinoids (e.g., WIN-55)<\/span><\/p>\n

2.7.3.6 Inhalation<\/strong><\/span>
\n 2.7.3.6.1 Oral inhalation<\/strong><\/span>
\n 2.7.3.6.1.1 Flowers (aka “bud”), hash, or other concentrates are combusted or vaporized (aka “vaped”) to decarboxylate (activate) with heat during consumption<\/span>
\n 2.7.3.6.1.2 Oils can also be pre-decarboxylated (activated) and delivered as a mist from a heated atomizer (vape pen)<\/span>
\n 2.7.3.6.2 Oral\/heated and nasal\/unheated combined inhalation<\/strong>
\n(e.g., Terpz inhalation system<\/a>)<\/span>
\n 2.7.3.6.3 Oral or nasal inhalation of terpenes<\/strong><\/span>
\n 2.7.3.6.3.1 oral<\/span>
\n 2.7.3.6.3.2 nasal<\/span><\/p>\n

2.7.3.7 Edibles – Infused Products<\/strong>
\n(Note: may be pre-activated (A), heated before consumption; or inactive (I)<\/span>)
\n 2.7.3.7.1 Raw plant<\/strong><\/span>
\n 2.7.3.7.1.1 edible<\/span>
\n 2.7.3.7.1.1.1 juiced<\/span>
\n 2.7.3.7.1.1.1 salad greens<\/span>
\n 2.7.3.7.1.1.1 fresh (same day harvest)<\/span>
\n 2.7.3.7.1.1.1 storage period<\/span>
\n 2.7.3.7.2 Food and drinks<\/strong><\/span>
\n 2.7.3.7.2.1 infused liquids<\/span>
\n 2.7.3.7.2.2 infused solids<\/span>
\n 2.7.3.7.3 Capsule or pill<\/strong><\/span><\/p>\n

2.7.3.8 Sublingual<\/strong><\/span>
\n 2.7.3.8.1 Spray<\/span>
\n 2.7.3.8.2 Tincture<\/span>
\n 2.7.3.8.3 Candy<\/span><\/p>\n

2.7.3.9 Topical<\/strong><\/span>
\n 2.7.3.9.1 oil<\/span>
\n 2.7.3.9.2 lotion<\/span>
\n 2.7.3.9.3 cream<\/span>
\n 2.7.3.9.4 spray (e.g., for topical relief of neuropathy, arthritis)<\/span><\/p>\n

2.7.3.10 Transdermal patch<\/strong><\/span><\/p>\n

2.7.3.11 Vaginal suppository<\/strong><\/span><\/p>\n

2.7.3.12 Rectal suppository<\/strong><\/span><\/p>\n

2.7.3.13 Intravenous injection<\/strong><\/span><\/p>\n

2.7.3.14 Timing of Administration<\/strong><\/span>
\n 2.7.3.14.1 time-released capsule or caplet<\/span>
\n 2.7.3.14.2 when administered<\/span>
\n 2.7.3.14.2.1 same time each day in circadian rhythm<\/span>
\n 2.7.3.14.2.2 with meal<\/span>
\n 2.7.3.14.2.3 before meal<\/span>
\n 2.7.3.14.2.4 after meal<\/span>
\n 2.7.3.14.3 interaction with other medicines and supplements<\/span>
\n 2.7.3.14.4 interaction with types of food and drink<\/span><\/p>\n

2.7.3.15 MOA, MOE, and TOA are matched to subject’s medical condition and purpose of study<\/strong><\/span>
\n 2.7.3.15.1 Method of Administration<\/strong><\/span>
\n 2.7.3.15.2 Method of Extraction<\/strong><\/span>
\n 2.7.3.15.3 Timing of Administration<\/strong><\/span><\/p>\n

2.7.4 Data collection and metrics (examples):<\/strong><\/span>
\n 2.7.4.1 Qualitative data<\/span>
\n 2.7.4.1.1 Self-reporting survey (with open-ended questions)<\/span>
\n 2.7.4.1.2 In-depth subject interviews<\/span>
\n 2.7.4.1.3 Field research<\/span>
\n 2.7.4.1.4 Search of relevant anecdotal literature<\/span>
\n 2.7.4.2. Quantitative data<\/span>
\n 2.7.4.2.1 Self-reporting survey (with quantitative questions)<\/span>
\n 2.7.4.2.2 Physician reporting<\/span>
\n 2.7.4.2.3 Medical records search<\/span>
\n 2.7.4.2.4 Data analysis from other studies<\/span>
\n 2.7.4.2.5 Epidemiological<\/span>
\n 2.7.4.2.6 Meta-analysis<\/span>
\n 2.7.4.2.7 Search of relevant peer-reviewed published studies<\/span>
\n 2.7.4.2.8 Serum metabolites measured<\/span><\/p>\n

2.8.0 Data analysis<\/span><\/strong><\/p>\n

2.8.1 Qualitative Data<\/strong><\/span>
\n 2.8.2 Quantitative Data<\/strong><\/span>
\n 2.8.3 Statistical analysis<\/strong><\/span>
\n 2.8.3. Common statistical tests and procedures (per Wiki) include:<\/span>
\n 2.8.3.1 Analysis of variance (ANOVA)<\/span>
\n 2.8.3.2 Chi-squared test<\/span>
\n 2.8.3.3 Correlation<\/span>
\n 2.8.3.4 Factor analysis<\/span>
\n 2.8.3.5 Mann\u2013Whitney U<\/span>
\n 2.8.3.6 Mean square weighted deviation (MSWD)<\/span>
\n 2.8.3.7 Pearson product-moment correlation coefficient<\/span>
\n 2.8.3.8 Regression analysis<\/span>
\n 2.8.3.9 Spearman’s rank correlation coefficient<\/span>
\n 2.8.3.10 Student’s t-test<\/span>
\n 2.8.3.11 Time series analysis<\/span><\/p>\n

2.9.0 Discussion<\/span><\/strong>
\n 2.9.1 Discussion should reflect an understanding of the complexities of cannabis research<\/span>
\n 2.9.2 Comments:<\/span><\/p>\n

2.10.0 Findings<\/span><\/strong>
\n 2.10.1 Findings noted should be supported by the data<\/span>
\n 2.10.2 Comments:<\/span><\/p>\n

2.11.0 Interpretation<\/span><\/strong>
\n 2.11.1 Interpretation should be supported by the data<\/span>
\n 2.11.2 Comments:<\/span><\/p>\n

2.12.0 Conclusion<\/span><\/strong>
\n 2.12.1 Conclusions should be supported by the data<\/span>
\n 2.12.2 Comments:<\/span><\/p>\n

<\/h1>\n
\n

 <\/p>\n

Table of Contents<\/span><\/a> \u00a0 \u00a0 Introduction<\/a>\u00a0\u00a0\u00a0\u00a0 Checklist<\/a>\u00a0\u00a0\u00a0\u00a0 Compilation List<\/a>
\nFull View<\/a>\u00a0\u00a0\u00a0\u00a0 Printable Files<\/a> \u00a0 \u00a0 Guest Blog<\/a><\/span><\/strong><\/em><\/p>\n

\u00a0<\/span><\/strong><\/h1>\n

[\/vc_column_text][\/vc_column][\/vc_row]<\/p>\n<\/section>","protected":false},"excerpt":{"rendered":"

[vc_row][vc_column][vc_row_inner][vc_column_inner width=”1\/1″][vc_column_text] Table of Contents \u00a0 \u00a0 Introduction\u00a0\u00a0\u00a0\u00a0 Checklist\u00a0\u00a0\u00a0\u00a0 Compilation List Full View\u00a0\u00a0\u00a0\u00a0 Printable Files \u00a0 \u00a0 Guest Blog \u00a0 beta version [\/vc_column_text][\/vc_column_inner][\/vc_row_inner][vc_column_text] 2.0.0 CMAP\u2122 Checklist: for Cannabis Research in Human Subjects (Appendix A) 2.1.0 Purpose of the Study 2.1.0.1 Exploratory 2.1.0.2 To prove a hypothesis 2.2.0 Sponsor 2.2.0.1 Institution 2.2.0.2 Private study 2.2.0.3 Not […]<\/p>\n","protected":false},"author":1,"featured_media":281,"parent":8,"menu_order":2,"comment_status":"open","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-83","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"http:\/\/cmap420.com\/wordpress\/wp-json\/wp\/v2\/pages\/83","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/cmap420.com\/wordpress\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/cmap420.com\/wordpress\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/cmap420.com\/wordpress\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/cmap420.com\/wordpress\/wp-json\/wp\/v2\/comments?post=83"}],"version-history":[{"count":22,"href":"http:\/\/cmap420.com\/wordpress\/wp-json\/wp\/v2\/pages\/83\/revisions"}],"predecessor-version":[{"id":1280,"href":"http:\/\/cmap420.com\/wordpress\/wp-json\/wp\/v2\/pages\/83\/revisions\/1280"}],"up":[{"embeddable":true,"href":"http:\/\/cmap420.com\/wordpress\/wp-json\/wp\/v2\/pages\/8"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/cmap420.com\/wordpress\/wp-json\/wp\/v2\/media\/281"}],"wp:attachment":[{"href":"http:\/\/cmap420.com\/wordpress\/wp-json\/wp\/v2\/media?parent=83"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}